The DaAn Gene nCov RNA Kit is used for the in vitro qualitative detection of novel coronavirus (2019-nCoV) ORFlab and N gene in the throat swabbed sputum specimens of suspected pneumonia patients infected by novel coronavirus, suspected clustering cases, and others needing diagnosis or differential diagnosis for novel coronavirus.
Detection of novel coronavirus RNA shall meet the requirements of related guideline,clinical
diagnostic/disease control and other documents, so as to carry out the regulations on biosafety. The
detection results of this kit are for clinical reference only and should not be used as the sole criteria for
clinical diagnosis. It is recommended to conduct a comprehensive analysis on the condition in
combination with the clinical manifestations of the patient and other laboratory tests.
This kit is based on one-step RT-PCR technique. In practice, 2019 Novel Coronavirus (2019-nCoV)
ORF1ab and N genes are selected as amplification target regions. Specific primers and fluorescent
probes are designed for the detection of 2019 Novel Coronavirus RNA in the specimens. This kit also
includes an endogenous internal standard detection system, which is used for monitoring over the
processes of specimen collection, RNA and PCR amplification, thereby reducing false negative results.
- The analytical sensitivity of this kit is 500 copies/ml
- CV < 5%
- No cross-reaction with other pathogens
- –20 +/- 5℃ for 6 months
Frequently Asked Questions
Q: Where has this test been used?
A: This test has been used in Europe, The Middle East, Africa, and South East Asia.
Q: What machines work with this test?
A: The test can be processed by any of: ABI 7500, LightCycler480, AGS4800, Bio-Rad CFX96
- 1x NC(ORF1ab/N) PCR reaction liquid A 900 μl/ tube 1 – Main constituents: specific primers, probes, tris(hydroxymethyl)aminomethane-hydrochloric acid buffer
- 1x NC(ORF1ab/N) PCR reaction liquid B 200 μl/ tube – Main constituents: Hot start Taq DNA polymerase, c-MMLV reverse transcriptase